Auramine O CAS 2465-27-2

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Auramine O CAS 2465-27-2

CAS: 2465-27-2
Molecular Formula: C17H22ClN3
Molecular Weight: 303.83
EINECS: 219-567-2
HS Code: 3204 13 00

Synonyms: PYOCTANINUM AUREUM; PYOCTANUNUM AUREUM; PYOKTANIN YELLOW; PYKOTANNIN; AURAMINE O, CERTIFIED; AURAMINE O, CERTIFIED (C.I. 41000); AURAMINE O, FOR MICROSCOPY; BASIC YELLOW 2

What is Auramine O CAS 2465-27-2?

Auramine O CAS 2465-27-2 is a basic yellow fluorescent dye (C.I. 41000), also known as Basic Yellow 2 or Auramine hydrochloride, commonly employed as a stain in microbiology and histology for visualizing specific microorganisms under fluorescence microscopy.

Specification

Item Value
Appearance Yellow to ochre-yellow Powder/Solid
Melting Point y>250 °C (dec.)(lit.)
pka 9.8, 10.7(at 25℃)
pH 6-7 (10g/l, H2O, 20℃)
Storage Condition Inert atmosphere, Room Temperature

Feature

  • Strong Fluorescence: Produces bright yellow-green glow under UV light for easy visualization.
  • Acid-Fast Affinity: Binds strongly to mycolic acids in bacterial cell walls.
  • Certified Grade: Available in Biological Stain Commission-certified forms for reliable microscopy use.
  • Versatile Compatibility: Often combined with Rhodamine B in Truant auramine-rhodamine staining protocols.

Application

  • Tuberculosis Detection: Primary fluorescent stain for identifying Mycobacterium tuberculosis and other acid-fast bacilli in sputum or tissues.
  • Fluorescence Microscopy: Used in methods like Auramine O alone or auramine-rhodamine for low-magnification screening of acid-fast organisms.
  • Histology Staining: Applied in diagnostic assays, hematology, and histology for bacteria and coccidia visualization.
  • Textile Dyeing: Serves as a dye or intermediate for coloring paper, leather, and fabrics.
  • Research Tool: Employed in biological studies, including as a fluorescent Schiff reagent or for parasite detection.

Packaging

Mikem offers flexible packaging options, including 25 kg of fiber drums for liquid products and 15–25 kg PP/PE bags for powder products, all customizable to meet your specific needs.

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