What is Auramine O CAS 2465-27-2?
Auramine O CAS 2465-27-2 is a basic yellow fluorescent dye (C.I. 41000), also known as Basic Yellow 2 or Auramine hydrochloride, commonly employed as a stain in microbiology and histology for visualizing specific microorganisms under fluorescence microscopy.
Specification
| Item |
Value |
| Appearance |
Yellow to ochre-yellow Powder/Solid |
| Melting Point |
y>250 °C (dec.)(lit.) |
| pka |
9.8, 10.7(at 25℃) |
| pH |
6-7 (10g/l, H2O, 20℃) |
| Storage Condition |
Inert atmosphere, Room Temperature |
Feature
- Strong Fluorescence: Produces bright yellow-green glow under UV light for easy visualization.
- Acid-Fast Affinity: Binds strongly to mycolic acids in bacterial cell walls.
- Certified Grade: Available in Biological Stain Commission-certified forms for reliable microscopy use.
- Versatile Compatibility: Often combined with Rhodamine B in Truant auramine-rhodamine staining protocols.
Application
- Tuberculosis Detection: Primary fluorescent stain for identifying Mycobacterium tuberculosis and other acid-fast bacilli in sputum or tissues.
- Fluorescence Microscopy: Used in methods like Auramine O alone or auramine-rhodamine for low-magnification screening of acid-fast organisms.
- Histology Staining: Applied in diagnostic assays, hematology, and histology for bacteria and coccidia visualization.
- Textile Dyeing: Serves as a dye or intermediate for coloring paper, leather, and fabrics.
- Research Tool: Employed in biological studies, including as a fluorescent Schiff reagent or for parasite detection.
Packaging
Mikem offers flexible packaging options, including 25 kg of fiber drums for liquid products and 15–25 kg PP/PE bags for powder products, all customizable to meet your specific needs.